Vincenzo Montella’s side will be back in action when they take on Netherlands on Sunday evening.
The 2-0 win in the Europa League on Saturday will ensure the reigning champions remain in the mix for the trophy.
However, the draw will also see the return of a squad that has already played in the competition.
Napoli’s Marcelo Brozovic, who was suspended by Napoli after being found guilty of racist abuse, will return after being suspended from the tournament for a year after his arrest in March.
Matthias Sammer will return from a suspension after his side were denied a place in the final.
Vincenzio Montella will have his options limited in midfield with Mario Mandzukic and Mario Balotelli both suspended.
They will have to find a substitute to take their place.
Mario Mandzuka, who is expected to make his debut for the Netherlands, has been named on the bench.
Marco van Basten, who has not played since being rested for a Europa League game, will also be absent due to a knee injury.
Alvaro Morata will be the only player in the starting line-up that has not been injured, having been rested by Napolo on Saturday.
In attack, Antonio Rudiger, Mario Balzi and Lucas Perez will be joined by the likes of Paulo Dybala, Mario Gabbiadini, Andrea Pirlo, Andrea Barzagli, Alessandro Florenzi, Paulo Dybo, Roberto Baggio, Roberto Firmino, Paulo Di Vaio and Graziano Pelle.
Miguel Brito will be out injured after being replaced by Gianluca Di Marzio.
There will be no substitutes available for either side and the game will be played in Stadio Olimpico.
Tales of a Napoli side that has lost its way have been rife for some time now and it will be a test for the current crop of players.
A win will give the Rossoneri a first place in Group F and they will be in a position to claim the Europa prize.
Juventus have a huge advantage over the Nerazzurri in the table and it is expected that they will win the tie.
Dinamo Zagreb, who have lost four games in a row, have the advantage in the Champions League, having drawn all four games they have played so far.
Milan will also benefit from the draw because they have lost three of their last four Champions League games.
Their next home game will come against Atletico Madrid on Tuesday.
Elsewhere, the Italian giants will face Atletico Zaragoza in a second-leg at the Stadios Olimpic in the city of Marbella, with a possible return home for the Nerazurri.
Sampdoria will be without Leonardo Bonucci for the first leg, with Andrea Barzunini the only other player to miss out due to suspension.
Graziano Pellè will also miss the tie due to an ankle injury.
The Nerazzurs will also need to take on Real Madrid in the first round of the Champions Leagues with the home side needing to win at home to Monaco in order to qualify.
Roma have not lost in their last three Champions League encounters against Juventus and it would not be surprising if they beat the Neras to reach the last eight.
Riccardo Montolivo has made his first start of the season, with Leonardo Bonaventura also in the side, while Andrea Barcelo, who played for the hosts last time out, has started in place of Alessandro Del Piero.
With all of the squads already booked, there is no point playing in extra time.
Both clubs will have their legs under their belt in the group stages and they are the favourites to reach their first Champions League final.